Chromatography Forum

Hi all,

I have no experience on ion exchange chromatography. I have the method, the column and other things. But I have no idea how to clean the system after running, in case of one night stop and several days.

Thanks for any help.

In principle, not really any different from ion-pair:
- follow the column manufacturer's recommendation for a storage buffer.
- it takes about 10 column volumes of buffer to get complete physical wash-out of a column.
- allow longer equilibration when going from a strongly bound ion (e.g., sulfate) to a weakly bound ion (e.g., chloride)
- try not to leave chloride-containing buffers stagnant in the system overnight.
- watch out for microbial growth -- filter your buffers daily and prepare fresh frequently.
- never run while holding pointed objects like scissors in your hand .

Thank you very much !