Flushing in IEC system

[thohry]

Hi all,

I have no experience on ion exchange chromatography. I have the method, the column and other things. But I have no idea how to clean the system after running, in case of one night stop and several days.

Thanks for any help.

[tom jupille]

In principle, not really any different from ion-pair:
- follow the column manufacturer's recommendation for a storage buffer.
- it takes about 10 column volumes of buffer to get complete physical wash-out of a column.
- allow longer equilibration when going from a strongly bound ion (e.g., sulfate) to a weakly bound ion (e.g., chloride)
- try not to leave chloride-containing buffers stagnant in the system overnight.
- watch out for microbial growth -- filter your buffers daily and prepare fresh frequently.
- never run while holding pointed objects like scissors in your hand .

[thohry]

Thank you very much !