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Can I separate xylene isomers (o, m, p) by HPLC-UV? Methods?

http://www.chromforum.org/viewtopic.php?t=16316

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Can I separate xylene isomers (o, m, p) by HPLC-UV? Methods?

Posted: Tue Apr 12, 2011 11:14 pm
by rodrigaoman
Hello, everyone.
Can I separate xylene isomers (o, m and p) by HPLC-UV?
Does anyone know a method?
If I use C18 column isocratic acetonitrile water 60:40, do you think retention times will be longer?

Any comments? It may help me.

Thanks,
Rodrigo

Re: Can I separate xylene isomers (o, m, p) by HPLC-UV? Meth

Posted: Wed Apr 13, 2011 9:38 am
by Alex Buske
Yes, you can. I once tried that to demonstrate the effect of low flow rates on resolution.
AFAIR I used a 3µm C18 (15cm) with methanol to get more or less baseline resolution at around 7 min.

Alex

Re: Can I separate xylene isomers (o, m, p) by HPLC-UV? Meth

Posted: Wed Apr 13, 2011 4:01 pm
by DSP007
Hi
Here, however, says that "ksilene separations is not well reproduced for all types of C18 sorbents. At C18 xylene isomers are poorly separated by compared to the phenyl sorbents and compared with a normal phase chromatography (on silicagel)."
A free translation from Russian :D
http://www.anchem.ru/forum/read.asp?id=2000
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