Can I separate xylene isomers (o, m, p) by HPLC-UV? Methods?

[rodrigaoman]

Hello, everyone.
Can I separate xylene isomers (o, m and p) by HPLC-UV?
Does anyone know a method?
If I use C18 column isocratic acetonitrile water 60:40, do you think retention times will be longer?

Any comments? It may help me.

Thanks,
Rodrigo

[Alex Buske]

Yes, you can. I once tried that to demonstrate the effect of low flow rates on resolution.
AFAIR I used a 3µm C18 (15cm) with methanol to get more or less baseline resolution at around 7 min.

Alex

[DSP007]

Hi
Here, however, says that "ksilene separations is not well reproduced for all types of C18 sorbents. At C18 xylene isomers are poorly separated by compared to the phenyl sorbents and compared with a normal phase chromatography (on silicagel)."
A free translation from Russian
http://www.anchem.ru/forum/read.asp?id=2000