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THF for GPC
Posted: Thu Mar 10, 2011 7:37 pm
by push
Which THF do you recommend for GPC. It sholud contain 0.2g/l BHT. Does THF HPLC grade of Merck, Baker, etc contain BHT or I need to add it to the solvent?
Re: THF for GPC
Posted: Fri Mar 11, 2011 1:45 am
by tom jupille
Re: THF for GPC
Posted: Fri Mar 11, 2011 9:50 am
by gstaepels
I use THF p.a. from Merck. It contains 250 ppm BHT. Works fine with RI, not with ELSD
Regards
Gilbert
Re: THF for GPC
Posted: Mon Mar 28, 2011 10:16 am
by Dana
gstapels, what do you mean that the THF doesnt work fine with ELSD? I'm trying to determine an UV-stabilizer solved in THF with ELSD but get high noise, could this be one of the problems?
Re: THF for GPC
Posted: Tue Mar 29, 2011 9:12 am
by gstaepels
Whebn I use THF p.a. from Merck on my ELSD (Alltech 3300 ELSD) the baseline is too noisy. I have tried several brands of THF and the one which gives the least noise on the ELSD is the one from Biosolv (ULC/MS grade). So I use this for GPC, but after the sequency has finished, I flush the GPC columns with THF p.a., because you do not want peroxides formed due to unstabilized THF.