THF for GPC

[push]

Which THF do you recommend for GPC. It sholud contain 0.2g/l BHT. Does THF HPLC grade of Merck, Baker, etc contain BHT or I need to add it to the solvent?

[tom jupille]

http://www.mallbaker.com/Americas/catal ... language=E

[gstaepels]

I use THF p.a. from Merck. It contains 250 ppm BHT. Works fine with RI, not with ELSD

Regards
Gilbert

[Dana]

gstapels, what do you mean that the THF doesnt work fine with ELSD? I'm trying to determine an UV-stabilizer solved in THF with ELSD but get high noise, could this be one of the problems?

[gstaepels]

Whebn I use THF p.a. from Merck on my ELSD (Alltech 3300 ELSD) the baseline is too noisy. I have tried several brands of THF and the one which gives the least noise on the ELSD is the one from Biosolv (ULC/MS grade). So I use this for GPC, but after the sequency has finished, I flush the GPC columns with THF p.a., because you do not want peroxides formed due to unstabilized THF.