Monitoring Alkaline Hydrolysis using HPLC

[gavin.fernandes]

Hi All,

I'm a conducting a process in the lab where I am added water to wheat protein and then alkaline hydrolysing the wheat protein with Sodium Hydroxide of a final concentration of 20mM.

I wish to monitor the reaction and hydrolysis of the wheat protein over time. I'm trying to obtain a specific molecular weight.

Does anyone have any experience with trying to monitor alkaline hydrolysis using HPLC? If so could you please send me the columns used, methods, mobile phases, etc.

Regards

Gavin

[InfraredRoses]

• Has this hydrolysis worked so far? I can't imagine much hydrolysis at a final conc of 20mM. Anyways it's not so much the hydrolysis you should be thinking about, but more the product that you wish to analyze. I assume you will be making a protein and some smaller protein/peptides. You need a lot more information to start to answer your question.
  1) what size do you expect the product(s) to be?
  2) are you repeating known work or trying an experiment?