Chromatography Forum

Dear all,
Recently I purchased Chiral gels equivalent to Daicel AS-H / AD-H, 5 micron from Lanzhou Ateo, China, to pack in to self packing columns of 10mm/20mm Id.
Unfortunately, they denied the packing conditions, after supplying gels.
Can any one, help us by providing Bulk density to prepare slurry gel, pushing solvents.
Best regards,
A.L.N. MURTHY

So I will assume that your packing material is just a coated silica (or a material bound to silica)... ? If so, then any ordinary column packing technique should be fine. Keep an eye on the pressure as you may want to keep it lower than normal if it is a non-covalently bound material.

I normally use ethanol or IPA as the slurry solvent. The slurry concentration can be determined once you know the packing density of the material (ask the manufacturer). *The last silica based chiral column I packed was around 0.7 grams/ml. If you do not know the density, then you can also just start with a slurry of say ~ 500 grams/ 1 liter. Depending on the type of column packing system you have just remember to get rid of all gas/air before starting and use a solvent system that is appropriate for your material. In my example above, you could use a mixture of Methanol (or Ethanol) and IPA to pack the column.

Dear Sir,
Thank you for much for your valuable info.
Best regards,
A.L.N. MURTHY