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prebiotics analysis

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

5 posts Page 1 of 1
Hello:

I need to analyze prebiotics in infant formulas, but I need to know if I should buy a Dionex System or could I buy any other HPLC system.
What configuration do you recommend the instrument should have? (pump, detector, autosampler, etc)
Does anybody have information how to do this analysis?

Thanks in advance for your help.

Best regards.

No one here knows what you mean by the word "prebiotics." Could you be more specific?
Mark Tracy
Senior Chemist
Dionex Corp.

Googggllleee? Yes Google?

Mark here what comes when you punch the word on Google:

prebiotics (pree.bye.AW.tiks) n. Nondigestible food substances that improve health by stimulating the growth or activity of beneficial bacteria within the colon.
—prebiotic adj.


Example Citation:


Although evidence indicates that extra doses of "good" bacteria, such as lactobacilli and bifidobacteria, can relieve inflammation and restore harmony, delivering them alive to the colon is not easy. The bacteria can be grown in yoghurt cultures or freeze-dried in capsules, but there is no guarantee that the products in health shops and supermarkets contain the optimum number of bacteria, nor that they will survive the hazardous passage through churning stomach acids and digestive enzymes to the gut. And as Professor Glenn Gibson, a microbiologist at the school of food biosciences at Reading University, explains: "Unlike probiotic bacteria, prebiotic carbohydrates are not destroyed when cooked."

Professor Gibson and a Belgian colleague, Dr Marcel Roberfroid, of Louvain University, Brussels, coined the term "prebiotics" in 1995.

Prebiotic carbohydrates, known as oligosaccharides, are found naturally in certain fruit and vegetables, including bananas, asparagus, garlic, wheat, tomatoes, Jerusalem artichoke, onions and chicory. They are not digested but go straight to the gut where they are seized on by good bacteria, stimulating the bacteria's growth.

"Because you have more chances of getting prebiotics to the large intestine, you see a bigger response than you would possibly expect with a probiotic," says Gibson.
—Anne Woodham, "A good gut feeling," The Times of London, June 11, 2003



Earliest Citation:

An alternative approach to the manipulation of the gut microflora is the use of prebiotics. These are food ingredients that selectively target the colon and may beneficially affect the host by stimulating the growth or activity of specific resident bacteria. Again, the goal is to realise potential health benefits. Dr Glenn Gibson of the UK's Medical Research Council, Dunn Clinical Nutrition Centre, discussed the functional use of prebiotics, referring to the non-digestible oligosaccharides and their influence on bifidobacteria. A number of health-promoting properties are associated with bifidobacteria including an anti-bacterial effect, production of vitamins (particularly of the B Group) and the production of certain immunomodulators that may promote immunological attack against malignant cells.
—Liz Tuley, "Functional foods: the technical issues," Food Manufacture, April 1995

Thanks for the references. Actually I was hoping that Alex T would tell us what he thinks it means, and perhaps a reference to why he thinks Dionex is the only way to go.

Dionex does have some good products for analysis of oligosaccharides, and if you want to use our CarboPac columns, you might be better off using our systems too. (What did you expect me to say?) Since these columns use NaOH/NaOAc for a mobile phase, specifying a pump, autosampler and pulsed amperometric detector (Au electrode) is a bit of an exercise. Not impossible, just tricky. The main thing is materials of construction should be highly base-resistant and tolerant of high dissolved salts. Then see if someone has worked out an optimum pulse-waveform on their detector.

Alex T really needs to talk to various company representatives about this, and this forum is not the best place for that.
Mark Tracy
Senior Chemist
Dionex Corp.
Hello:

I need to analyze prebiotics in infant formulas, but I need to know if I should buy a Dionex System or could I buy any other HPLC system.
What configuration do you recommend the instrument should have? (pump, detector, autosampler, etc)
Does anybody have information how to do this analysis?

Thanks in advance for your help.

Best regards.
Hello,

If you are talking about fructans (which are prebiotic oligosaccharides), there are several AOAC methods dealing with this subject, using Dionex instrument. However the latter method published (999.03) is an enzymatic-spectrophotometric, so Dionex is not needed anymore. This method is based on a Megazyme kit http://www.megazyme.com/booklets/KFRUC.pdf

Regards
VG
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