Tailing high and increases with injection volume
Posted: Wed Jan 19, 2011 8:45 am
A question about tailing: This is for assay testing. Normal concentration and injection volume (5uL) gives 2.119 tailing. When injection amount changed by injection volume (uL) it gives inj.vol/tailing values 2/1.556, 3/1.772, 5/2.119, 7/2.316, 10/2.617. Clearly at normal condition tailing is too high, when injection amount increased tailing also increases. Is this not acceptable (for a good reliable assay method)? What are the risks? Does this variation suggest column overloading?