UV vis vs fluorescence detector for vitamin E analysis

[krisradh1432]

Hi HPLC experts,

Is there any relationship between fluorescence and UV detector for vitamin E determination in normal phase HPLC.
This problem arise because the fluorescence HPLC is currenctly using by another researcher (may take few weeks for her to complete) while UV HPLC is unused.So I planned to evaluate the efffect of few variables e.g. temp and time to the tocols in my sample using HPLC- UV/Vis. After that, I wish to use fluorescence HPLC for method optimization.
Can I follow this way??.
Will there be a difference in amount regardless the tocols concentration

PLease advise me.
Thank You

[JGK]

Our Standard method for determination of Tocopherols and Tocotrienols in oils uses UV detection, I have no idea about fluorescence.

However, if each method is calibrated correctly you should obtain similar results from the same sample from both assays .