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Replace phosphate buffer for LC prep
Posted: Fri Feb 18, 2005 1:25 pm
by Provetech
I want to purify a product (amino group) which is analysed with a phosphate buffer ph:4,5 what is the nearest volatile buffer i could use to replace it for a preparative system.
Thanks
Posted: Sat Feb 19, 2005 3:20 am
by Uwe Neue
Use ammonium acetate at pH 4.5, and you have a real buffer (phosphate has no buffering capacity at pH 4.5). In order to prepare the buffer, use ammonium acetate, and then add acetic acid until the pH is at 4.5.
Posted: Sat Feb 19, 2005 11:24 am
by Provetech
Will i get volatile residues in the collected solution which will be eliminated on rotavapor or lyophilisated ?
Thanks
Posted: Sat Feb 19, 2005 4:43 pm
by Uwe Neue
It is reasonably volatile. Try it out! You will get faster results at elevated temperature, if your compound lets you do that.
Posted: Sun Feb 20, 2005 7:38 pm
by Provetech
Thank you very much, i will try soon to compare results for the separation...