Replace phosphate buffer for LC prep

[Provetech]

I want to purify a product (amino group) which is analysed with a phosphate buffer ph:4,5 what is the nearest volatile buffer i could use to replace it for a preparative system.
Thanks

[Uwe Neue]

Use ammonium acetate at pH 4.5, and you have a real buffer (phosphate has no buffering capacity at pH 4.5). In order to prepare the buffer, use ammonium acetate, and then add acetic acid until the pH is at 4.5.

[Provetech]

Will i get volatile residues in the collected solution which will be eliminated on rotavapor or lyophilisated ?
Thanks

[Uwe Neue]

It is reasonably volatile. Try it out! You will get faster results at elevated temperature, if your compound lets you do that.

[Provetech]

Thank you very much, i will try soon to compare results for the separation...