Solubility and Mobile phase

[deleon]

Hi!!

I'm working on a method and I may have some components that could left behing in the column so I'm trying to wash the column but I'm not sure that they are soluble in the mobile phase (I'm want to use ACN/IPA to wash the column before going back to original conditions).
Is it really a problem if those components are not soluble in ACN/IPA? Thanks!!
Dorcas

[danko]

If they’re not soluble in the mixture you’d like to apply, you just haven’t achieved anything (you’re still where you started). I’d prefer to have some water in the mixture – f.x. 10%. But there really isn’t a universal cleaning procedure that resolves all thinkable contamination problems. More structured methodology would be to find out what the possible contamination is and then apply the right solvent in order to clean/restore the column.

Best Regards

[ym3142]

Interesting!

if you start with 0.1mg/ml=100ng/ul solution and inject 10 ul to the column, had 1% carry over. equal to 10ng retained by the column.

If you have a mobile phase which has 10ng/mL solubility, therefore, it only take you one mL mobile phase to dissolve and wash the carryover off the column.

If a chemical X has only 10ng/mL solubility we will say chemical X is insoluble in this mobile phase. But, the calculation of above shows the mobile phase should easily remove this chemical in a column.

At least, we know it much more complicated to clean a colmn than solubility. And it does not require a lot solubility, if needed at all