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L-carnitine can be analyzed by HPLC-UV ?
Posted: Tue Aug 24, 2010 2:43 pm
by Nuunoo
Dear All,
It's my first topic to be posted, I'm trying to developed method L-Carnitine by HPLC-UV. Anyone have some advice.
Posted: Wed Aug 25, 2010 2:39 am
by SIELC_Tech
Here are few UV methods for analysis of similar compounds:
http://www.sielc.com/application_087.html
http://www.sielc.com/application_114.html
You need mixed-mode cation-exchange column in combination with UV-transparent mobile phase. You can see carbonyl in your molecule at 195-210 nm. Contact me if you have questions
Posted: Wed Aug 25, 2010 4:49 am
by bisnettrj2
Something to be aware of is the degradation product of L-carnitine having a greater UV response than L-carnitine. Here's a paper regarding the HPLC analysis of L-carnitine and the degradation product:
http://www.ncbi.nlm.nih.gov/pubmed/10933524
Posted: Wed Aug 25, 2010 2:46 pm
by Nuunoo
Thanks to All, I'm trying to analyse by column ODS-C18 and
I used mobile phase ion-pair pH 2.4 : MeOH , standard it's O.K. but I found the large peak in font of peak L-carnitine while run the samples (fruit juice).
Could you please to suggestions how to separate.
Posted: Wed Aug 25, 2010 7:57 pm
by bisnettrj2
Depends. What are the details of your mobile phase / column / system / etc? Can you post a chromatogram?
Posted: Thu Aug 26, 2010 10:53 pm
by unmgvar
you might need to improve the sample clean up as well,
Have you thought of HILIC for this separation?
Posted: Sun Aug 29, 2010 2:59 pm
by Nuunoo
Thanks to all for suggestion. now, I've to change mobile phase and column follow this paper. It's O.K.
http://www.ncbi.nlm.nih.gov/pubmed/10933524