L-carnitine can be analyzed by HPLC-UV ?

[Nuunoo]

Dear All,

It's my first topic to be posted, I'm trying to developed method L-Carnitine by HPLC-UV. Anyone have some advice.

[SIELC_Tech]

Here are few UV methods for analysis of similar compounds:

http://www.sielc.com/application_087.html
http://www.sielc.com/application_114.html

You need mixed-mode cation-exchange column in combination with UV-transparent mobile phase. You can see carbonyl in your molecule at 195-210 nm. Contact me if you have questions

[bisnettrj2]

Something to be aware of is the degradation product of L-carnitine having a greater UV response than L-carnitine. Here's a paper regarding the HPLC analysis of L-carnitine and the degradation product:

http://www.ncbi.nlm.nih.gov/pubmed/10933524

[Nuunoo]

Thanks to All, I'm trying to analyse by column ODS-C18 and
I used mobile phase ion-pair pH 2.4 : MeOH , standard it's O.K. but I found the large peak in font of peak L-carnitine while run the samples (fruit juice).
Could you please to suggestions how to separate.

[bisnettrj2]

Depends. What are the details of your mobile phase / column / system / etc? Can you post a chromatogram?

[unmgvar]

you might need to improve the sample clean up as well,

Have you thought of HILIC for this separation?

[Nuunoo]

Thanks to all for suggestion. now, I've to change mobile phase and column follow this paper. It's O.K.

http://www.ncbi.nlm.nih.gov/pubmed/10933524