Ammonia by HPLC?

[HPLC_Help]

Is HPLC a good way to measure ammonia? What kind of detector would work? RI?

What about GC? Would that be better?

Or is there a better method altogether.

I'm monitoring ammonium usage during a biological reaction.

Thank you for pointing me in the right direction!

H_H

[oscarBAL]

Hello H_H; ammonia can be analyzed either by HPLC (using ion exchange) or GC; but sometimes and depending of your concentration; steam destillation and Titration could be easier solution; if your problem is sencibility you can also use Selective Ammonia electrodes or colorimetric method; it is necesary to have in mind that these tree methods are sencitive to volatil amines also but work easier and faster.

[Markus Laeubli, Metrohm]

Hallo HPLC_Help

oscarbal is absolutely right. Ion Chromatography, titration or ISE all work in their respective concentration ranges and sample matrix conditions. For hgher concentrations I would go for Titration / ISE. For lower concentration and/or amines around, IC will be the preffered method.

[Phycal]

Also can use the Nessler reagent and do it spectrophotometrically.

[praveenpaliwal]

Try Inertcap column for volatile amine u can get good responce not only for ammonia but other volatile amine also on GC-TCD

Praveen

[SIELC_Tech]

here is method for analysis of ammonia using Primesep mixed-mode column and ELSD detector. You can analyze non-UV active amines using this approach:
http://www.sielc.com/compound_005.html

Contact me if you have questions