which cyano column is most robust?

[jiang295]

i do not know which prep scale cyano colun is most durable and at reasonable price? thanks a lot.

[rwang]

prep cyano column doesn't sound like a very good idea:

1. Expensive (as with all prep columns)
2. Cyano columns don't live nearly as long (compared with C18)
3. Loadability is less (again compared with C18)

Feel free to correct me if I'm wrong

[bisnettrj2]

To second rwang's post...

From Uwe Neue in a previous Chromforum discussion:

viewtopic.php?t=11206&sid=bc5eca77f9127 ... 59e44d38ca

I personally would NEVER use a cyano column for prep applications. First, cyano ligands hydrolyze much easier than C18 ligands, second, cyano columns tend to be unstable (as Bruce has seen), and third, I expect the loadability to be much lower than that of a C18....

...My advise... is to get the literature from the manufacturers that are worth considering, and then COMPARE their products based on the described features, such as column stability and preparative loadability. Since prepartive columns are expensive, you want to make sure that you get your money's worth.

[jiang295]

that is great. thanks guys.
the reason I want to choose cyano column is its selectivity and less hydrophobility, which can make isocratic run possible.

To second rwang's post...

From Uwe Neue in a previous Chromforum discussion:

viewtopic.php?t=11206&sid=bc5eca77f9127 ... 59e44d38ca

I personally would NEVER use a cyano column for prep applications. First, cyano ligands hydrolyze much easier than C18 ligands, second, cyano columns tend to be unstable (as Bruce has seen), and third, I expect the loadability to be much lower than that of a C18....

...My advise... is to get the literature from the manufacturers that are worth considering, and then COMPARE their products based on the described features, such as column stability and preparative loadability. Since prepartive columns are expensive, you want to make sure that you get your money's worth.