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problem about quantifying a natural lipid compound

http://www.chromforum.org/viewtopic.php?t=13444

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problem about quantifying a natural lipid compound

Posted: Tue Jul 20, 2010 9:43 pm
by shahis77
I am trying to develop a method for quantification of a lipid compound in Human plasma.The compound is naturally present in human plasma.So I am confused even if I extract about 80 % how to calculate the results as it is already present in the plasma.

Posted: Tue Jul 20, 2010 10:49 pm
by Don_Hilton
From your brief descripion, I can think of two approaches. 1) addition of a labeled standard and 1) if no labeled standard is available, standard additions.
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