Chromatography Forum

Hello.

I have a question.

When you analize to find a detection limit on LC the analite should give at least 3 times the noise level.

my question is, that noise level must be considered only on the RT window of the compound or in the whole run time?

thanks for your help

Best regards
greetings from México

*that* is a large "can of worms". In my opinion, better to estimate DL statistically from the slope and standard error of the calibration line.