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Problem with Agilent 1200 detector

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
We use this for radioactive metabolite studies, the system is hooked up to Lab Logic's Laura software.
http://www.lablogic.com/displaysoftware.asp?name=laura

what I'm seeing now is that when I manually inject a sample, everything initialises as expected, but then the detector goes to "not ready" status (red light) and everything grinds to a halt. Interestingly, a message displays saying "injection failed".

I'm not sure if it's the detector or the manual injector's sensor causing the problem. someone will be coming out in the next few weeks to hook up another instrument for us, and I'm sure he'll know what the problem is, but I thought I would post it here first.

Can you try using it without the datasystem? Do you have the gameboy?

I'm not familiar with the software but I suspect that the detector is looking for a signal from the autosampler saying that the injection has been made. You will need to have a ttl contact closure switch on your manual injector. Most of them have this as a magnetic sensor which detects when you 'inject' your sample and puts out, usually, a ttl ground which needs to go be recieved by the detector so that it knows to begin reading and storing the signal.
3 posts Page 1 of 1

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