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Temperature range for ACE 300 C4 phase
Posted: Thu Jul 01, 2010 1:25 pm
by grumbledook
I am currently testing the 3 µm ACE 300 C4 RP. Unfortunately I wasn't able to find information about thermal stability for this particular phase. Can anyone comment on this? (I would like to run it routinely at 60 °C.)
Thanks in advance!
Posted: Thu Jul 01, 2010 6:04 pm
by danko
I've used this particular column at high temperature (55° C) once.
Just like all other silica based column materials it's stable at least up to 60° C. I wouldn't hesitate to apply even higher temperatures (e.g. 70° C).
Best Regards
Posted: Thu Jul 01, 2010 8:55 pm
by Tom Waeghe
I would recommend a temperature of 40C or less at pH 7-9, and would recommend a similar temperature range < 40C if working below pH 3 for best column lifetime.
Posted: Fri Jul 02, 2010 9:37 am
by grumbledook
OK, then I will stick to 40 °C. (Usually we analyze our samples at pH ~2.5)
Thanks!