Boronate Affinity Chromatography - Help Needed :(

[HydroSpark]

Message me please if you have any expertise in this area of chromatography. Solving my problem may result in compensation.

Thanks.

[danko]

Just a suggestion: It might be a good idea to shortly describe the conditions as well as the problem to be solved. I’m sure it’ll “attractâ€

[HydroSpark]

I am looking for a way of retaining the blood sample on a column of boronate beads. Currently the boronate I am using does not retain anywhere near enough of the blood sample on the column before washing. I have witnessed however boronate beads retaining far more of the blood sample on another type of bead which composition is not known to me.

Thanks.

[Andy Alpert]

By "blood sample" I presume you mean something like glycosylated hemoglobin (Hemoglobin A1c, etc.). There are two points to keep in mind:

1) All commercially-available boronate materials have a functional group that works well above pH 8.5 but is less effective at capturing glycosylated solutes at lower pH. What pH are you starting at?

2) If you are in fact analyzing glycosylated proteins, then the chromatography material should have a pore diameter wide enough to give them ready access to the interior. This should be 300 Ã… at least and preferably closer to 1000 Ã…. What's the physical characteristics of the material you're working with?

Andy Alpert
PolyLC Inc.
aalpert@polylc.com

[HydroSpark]

Hi, I've emailed you Andy. Thanks.

[HW Mueller]

Too bad that this is going underground.

[HydroSpark]

I am sorry, I just wanted to email directly.

Jessica