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About reversal peak
Posted: Mon May 24, 2010 7:38 am
by pheui
May people could accept the reversal peaks appeared between 1 to 5 min, and that is said as the peaks of solvent. While how about the reversal peak after the target peaks? How to explain this phenomenon? I am looking forward to your explaination. Thanks!
Posted: Mon May 24, 2010 9:04 am
by danko
You probably mean negative peaks? That's my assumption anyway.
If the stuff causing a negative peak absorbs less energy (my second assumption is that you're utilizing an absorbance detector) than the mobile phase does, at the given wavelength, you'll observe a negative peak, regardles of its retention time.
Best Regards
Posted: Mon May 24, 2010 2:09 pm
by unmgvar
Much more information from you is required to be able to help you.
I know of a IC application for example; where a negative peak at the tail of the main compound is exactly what you look for and try to quantify
What is your application?