About reversal peak

[pheui]

May people could accept the reversal peaks appeared between 1 to 5 min, and that is said as the peaks of solvent. While how about the reversal peak after the target peaks? How to explain this phenomenon? I am looking forward to your explaination. Thanks!

[danko]

You probably mean negative peaks? That's my assumption anyway.
If the stuff causing a negative peak absorbs less energy (my second assumption is that you're utilizing an absorbance detector) than the mobile phase does, at the given wavelength, you'll observe a negative peak, regardles of its retention time.

Best Regards

[unmgvar]

Much more information from you is required to be able to help you.

I know of a IC application for example; where a negative peak at the tail of the main compound is exactly what you look for and try to quantify

What is your application?