How to Install ELSD with HP1050 HPLC and Chemstation Ver.A

[Sallybeetle]

I want to install a Sedex 85LT ELSD on a HPLC system with HP1050 IEEE/GPIB connected components, and run on a Chemstation with version A.08(?). Is there a way to direct the ELSD signal into the Chemstation?

[Consumer Products Guy]

Yes. You need either an internal or external 35900 interface (an analog-to-digital converter).

[Jumpshooter]

Sally: what Analytes r you trying to quantify with your evaporative light scattering detector?

[Sallybeetle]

We have already developed and validated HPLC-ELSD methodologies for Gentamicin and Neomycin. We currently run HPLC-ELSD for these analytes on another HPLC system; but, due to work load and other considerations, we want to move the ELSD to the HP1050 / Chemstation HPLC system.

[HPLC_Help]

Yes. You need either an internal or external 35900 interface (an analog-to-digital converter).

Hi, Sallybeetle -

You'll also have to use the external contact closure cable that (on the HP1100, anyway) attaches to the back of the pump. This is to signal the instrument to start/stop runs and to autozero. In my experience, the contact closure cable didn't connect easily to the ELSD - you have to get the correct cables and connectors - but it works well after doing it.

Also, if you want to shut off gas after the run, you can get a Gas Mizer - a nifty little item sold by (I believe) Ticoscen. There are lots of posts on here from Tim at Ticoscen. He'll help you out.

H_H

[sscala]

Hi Sallybeetle,

We have already developed and validated HPLC-ELSD methodologies for Gentamicin and Neomycin.

would you mind to share some details about the methods for Gentamicin and Neomycin?

rgds.
S.Scala

[Consumer Products Guy]

1050 pump and autosampler modules each have contact closures (believe both as contact #2).