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- Posts: 6
- Joined: Wed Apr 09, 2025 2:17 pm
For reference I am using a Promochrom SPE-03 unit and Biotage High Capacity C18 SPE disks.
My extraction procedure is as follows:
Condition Disks
• Hexane>DCM>MeOH>Reagent water (pH 7)
• Load Sample at 25 mL/min
• Rinse 3x with 10 mL of reagent water
• Dry disks for 15 minutes at 5 L/min
Elute Disks
• Elute 3x with 15 mL of 50:50 DCM:EtOAc
• Elute 3x with 15 mL of 10:90 DCM:Hexane
Then I dry and concentrate the extracts as I would with a LLE.
I increased the nonpolar solvents in the extraction to try and pull the compounds of the disk, went from just DCM and EtOAc, to adding the 10:90 DCM:Hexane. I also increased the soak time of the disks from 1 minute to 3 minutes without any improvement.
Any ideas on how I can boost the recoveries of aldrin and heptachlor?
