Dear all,

we measure the alkaloid content of lupin seeds by GC-FID/MS.
For the identification and the quantification we have a se election of pure reference substances. Some are stored as salts (HCl or sulfate).
For quantifcation we dissolve the alkaloids (neutral and salts) in methanol.
For the spartein salt we obtanined a nice linear calibration curve.
However, I wonder whether the salt form might be a problem as the alkaloid salt might not be volatile or decompose. Has anyone eperience with the analysis of alkaloids by GC?

I think, that the nice linear calibration curve indicates that all of the spartein migrates through the column to the detector. What do you think about that?

Thanks for your help in advance.
Kind regards
Chris



Our method:
column DB-5 (30 m x 0.25 mm x 0.25 um) with He as the carrier gas (1 mL min-1; split ratio 1:20; injection port 250 °C; detection 300 °C)
Oven temperature program starts at 180 °C, 2 min isothermal, 5 °C min-1 to 300 °C, 10 min isothermal.