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Phospholipid extraction from tissues using Folch Method

Posted: Mon Apr 12, 2010 10:01 pm
by ssankella
Hello everyone
I am trying to extract phospholipids from tissues. Though I have the method from a paper its not in detail.
Can someone post the detailed protocol of Folch method to extract the phospholipids.

Any input will be greatly appreciated.

Regards
Sankella

Posted: Tue Apr 13, 2010 12:30 am
by bisnettrj2
Hopefully this is more than what you already have:

http://www.cyberlipid.org/extract/extr0005.htm#1

Posted: Tue Apr 13, 2010 5:56 pm
by JGK

Posted: Tue Apr 13, 2010 10:52 pm
by ssankella
Thank you bisnettrj2 and JGK.
That helps :)

Regards
Sankella