Phospholipid extraction from tissues using Folch Method

[ssankella]

Hello everyone
I am trying to extract phospholipids from tissues. Though I have the method from a paper its not in detail.
Can someone post the detailed protocol of Folch method to extract the phospholipids.

Any input will be greatly appreciated.

Regards
Sankella

[bisnettrj2]

Hopefully this is more than what you already have:

http://www.cyberlipid.org/extract/extr0005.htm#1

[JGK]

This may be of help http://www.unifr.ch/biochem/assets/file ... action.pdf

http://lipidlibrary.aocs.org/topics/extract2/file.pdf

[ssankella]

Thank you bisnettrj2 and JGK.
That helps

Regards
Sankella