stress study/specificity studies in method validations

[bhaskarac]

Can anybody share what is the good practice to follow to forcibly degrade the compound which are not solble in aquious solutions. Is it good idea to use methanolic NaoH, methanolic acids for degradation studies? If not what is the good practice.

Thanks in advance,

Bhaskar

[maris]

Acids and bases act as acids and bases only in aqueous media, so, presence of some amount of water in order to obtain "acidic" or "basic" degradation is necessary.

Using of Methanolic solution has some limitations: You have to be sure that there is no intetraction between your molecule , corresponding degradation products and methanol ( esterification for example). Otherwise you can find "new" degradation products which don't really related to your substance.
Some of may collegues use solid-solid (sometimes it's much easier to mix fine powders of the substance and NaOH) or solid - liquid (powdered substance + small amoumt of acid in suitable concentration) systems for stress degradation studies.

Good luck!