Bonded phase of Lichrosorb 60 B-select B column

[thohry]

Hi all,

I have an application that uses a Lichrosorb 60 B-select B column. I just want to use a similar column but do not know what is the phase : C18, C8 or CN.. Anyone knows what is that phase please help.

Thanks.

[bisnettrj2]

Point of clarification:

If I'm reading your question correctly, you have an appication that specifies a "Lichrosorb 60 B-select B column", but you don't have that specific column, so you want to use something similar, but you're unsure of the phase specified in the original application? If that's the case, perhaps some of the specifics from your application can give a clue as to the original phase (other details about the column, the analytes the application is intended for, and the mobile phase constituents)? Or maybe you can use the USP column equivalent tool to find a proper substitute?

http://www.usp.org/USPNF/columnsDB.html

[thohry]

Point of clarification:

If I'm reading your question correctly, you have an appication that specifies a "Lichrosorb 60 B-select B column", but you don't have that specific column, so you want to use something similar, but you're unsure of the phase specified in the original application? If that's the case, perhaps some of the specifics from your application can give a clue as to the original phase (other details about the column, the analytes the application is intended for, and the mobile phase constituents)? Or maybe you can use the USP column equivalent tool to find a proper substitute?

http://www.usp.org/USPNF/columnsDB.html

Thank you for the answer. My thinking is that, there are Lichrosorb C18, C8, CN that I can understand, but this case is 60 B Select, it's different.
Anyway, thanks again

[bisnettrj2]

Per the Merck website, I could only find the following:

LiChrospher® 60 RP-select B (5 µm)

"LiChrospher® RP-select B is a versatile traditionally produced spherical silica gel carriers with reversed phase properties and with the hydrophobicity of a C-8 phase. This carrier is very well suited for the separation of basic compounds, but with still good properties for the determination of neutral and acidic compounds."

So, not LiChrosorb, but LiChrospher, and it's a C8.

Also, on the USP website I posted earlier, there is a selection in the PQRI Database section for the LiChrospher 60 RP-Select B, and depending on the conditions you're using (pH, types of analytes you're looking for, etc.), the probable best-match column is a ProntoSIL 120-5 C8 SH.

[Alex Buske]

LichroSorb is irregular silica gel, LiChrospher is spherical. 60 is the pore size and Select-B the RP-Phase. Select B is something very special, the statement "with the hydrophobicity of a C-8 phase" doesn't imply that it really a C-8. IIRC there is some kind of amino-endkapping.
If you go for Prontosil, I would prefer the 60A-Material ProntoSIL 60-5 C8 SH.

Alex