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Separation of cresol isomers by HPLC
Posted: Sun Feb 21, 2010 5:39 pm
by praveenpaliwal
Hello,
How can I separate o,m and p-Cresol iosmers by HPLC is it possible or not.
I separated these three on GC (DB-wax column) but unfortunately the column has been damage and I need quick separation and arrival of new column will take time.
Can any body tell me is it possible to separate these three isomers on HPLC
Regards
Praveen
Posted: Sun Feb 21, 2010 7:23 pm
by bisnettrj2
An application note from Imtakt:
http://www.imtaktusa.com/site_media/fil ... TI025E.pdf
A referenced journal article for Normal Phase separation:
5.2. Husain, S.; Kunzelmann, P.; Schildknecht, H.; J. Chromatogr. (1977), 137, 53 - 60.
Posted: Wed Feb 24, 2010 4:22 pm
by praveenpaliwal
Unfortunately I don't have this type of packing material. Can i use Diacell Column Chiralcel AD-H or OJ-RH for this separation.
As per my concern the conventinal C-18 columns are not suitable for the separations.
Can I use the above described column's
Praveen
Posted: Wed Feb 24, 2010 8:41 pm
by bisnettrj2
I'd try the OJ-RH first, based on the what I read on Daicel's website for the OJ-RH:
Polysaccharaide Reversed Phase Chiral Columns
The reversed phase columns are patricularly [sp] suited to applications for the chiral separations of aqueous samples or ionisable compounds with aqueous eluents.
It can't hurt to try, but make sure you're not injecting anything onto the column that is discouraged by the manufacturer, and use their recommended mobile phases and flow rates.