Separation of cresol isomers by HPLC

[praveenpaliwal]

Hello,

How can I separate o,m and p-Cresol iosmers by HPLC is it possible or not.
I separated these three on GC (DB-wax column) but unfortunately the column has been damage and I need quick separation and arrival of new column will take time.
Can any body tell me is it possible to separate these three isomers on HPLC

Regards

Praveen

[bisnettrj2]

An application note from Imtakt:

http://www.imtaktusa.com/site_media/fil ... TI025E.pdf

A referenced journal article for Normal Phase separation:

5.2. Husain, S.; Kunzelmann, P.; Schildknecht, H.; J. Chromatogr. (1977), 137, 53 - 60.

[praveenpaliwal]

Unfortunately I don't have this type of packing material. Can i use Diacell Column Chiralcel AD-H or OJ-RH for this separation.
As per my concern the conventinal C-18 columns are not suitable for the separations.
Can I use the above described column's

Praveen

[bisnettrj2]

I'd try the OJ-RH first, based on the what I read on Daicel's website for the OJ-RH:

Polysaccharaide Reversed Phase Chiral Columns
The reversed phase columns are patricularly [sp] suited to applications for the chiral separations of aqueous samples or ionisable compounds with aqueous eluents.

It can't hurt to try, but make sure you're not injecting anything onto the column that is discouraged by the manufacturer, and use their recommended mobile phases and flow rates.