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- Posts: 8
- Joined: Sat Mar 01, 2025 12:40 am
My company has two laboratories with identical Shimadzu/Sciex LC-MS/MS triple quad instruments and identical LC columns. There is a test we run for a set of two analytes, and at the two laboratories, we find different retention times for these analytes. At lab 1, we have retention times of 1.75 mins and 2.75 mins; at lab 2, we have retention times of 2.5 mins and 3.0 mins, respectively.
Is this just a matter of e.g. different lengths of tubing between the LC and the MS, etc.? Or are these retention time differences indicative of some deeper discrepancy between the labs?
Thank you for any advice you can provide!