Converting mV.sec to mAu?

[Flowchemist]

Hi Everyone,

I've an HPLC system (Agilent) which is attached to an ELSD detector (Polymer Labs). The ELSD detector is connected to the system and gives peak area measurements in mAu.

However, I need to run some validation tests on the ELSD and the pass specifications (as per manufacturer instruction manual) are given in mV.sec.

How can I convert from mAu to mV.sec and vice versa? I can't seem to find the conversion factor anywhere. I would really appreciate any help on this. Thanks a lot!

Cheers,
Taylor

[Jumpshooter]

This is a conversion from an "electrical signal" as volts to "spectral signal" as absorbance units AU. The software in your DAD does this operation in the Black Box; however, you may derive the 'conversion factor' empirically by plotting the voltage signal against the absorbance value and acquiring the slope value for one of your Standard solutions. Set the Y-axis to readout as millivolts, run the standard sample; then set the y-axix to readout as AU, then run the STD. Plot 'millivolts' against 'AU"--The value of the slope would be your empirically derived conversion factor.