dead volume of the pump LC

[hoba]

Hello,

I'm working on high efficiency LC and I'd like to know the effect of the pump on the band width. What is the effect of each part (pressure sensor, pump head, piston etc..) and what is the dead volume of each?
I use Agilent 1200 with a binary pump.

Thanks

[bisnettrj2]

You posted in the wrong forum, to start. This should be in the liquid chromatography forum. I'd suggest you re-post there, for the proper consideration to this question.

Second, if you repost, please clarify what you mean by "the effect of the pu mp on the bandwidth". Do you mean gradient delay? Band broadening?

[DR]

moved to correct forum...

[thohry]

Hello,

I'm working on high efficiency LC and I'd like to know the effect of the pump on the band width. What is the effect of each part (pressure sensor, pump head, piston etc..) and what is the dead volume of each?
I use Agilent 1200 with a binary pump.

Thanks

The binary 1200 has dead volume from the mixer to the inlet of the column. It does not include pump, sensor... so it's not much.

[ljc]

It's spelled out clearly in the manual. About 1 mL. You can bypass the grad mixer in the 1200, and the mixer and pulse damper in the 1260 to le
ssen the dwell volume.

[HPLCCONSULT]

Per specification, 1200 series delay volume is ~ 300-400 ul (two different mixers used over the years) with the mixer removed. With the std tubing and the mixer installed (default mode) it averages around 800 ul. The mixer is the single largest contributor of extra volume in the system.

If you plan on running below 200 ul/min, then you should replace it with a smaller mixer as it is optimized for > 1 ml/min flow rates.