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- Posts: 83
- Joined: Wed Mar 25, 2020 8:52 am
We run ammonia on a waters h-class and we see a small jump in the baseline for every time the seal wash is operated. If we use 1min this jump is seen every 1min. Its a small jump but enough to impact loq if it happens at time where analyte peak is found. We use 3mM HNO3 in 70%meoh as eluent and I would prefer not to disable the seal wash for system durability comcerns. Seal wash is methanol 20-30% . The old hplc system we replaced did not use seal wash.
Anyone seen simmilar effects? Any possible solution?
Disabling seal wash for this method might be an option as we run very few samples around 100 annualy, thats why we did not get dedicated ic system. I am reluctant to not use seal wash for fear of pump wearing out.
I could add that this was seen even when system was new so its not likely a fault with a worn system.