Glycerin and carbohydrates in cream
Posted: Fri Dec 17, 2004 7:33 am
Hi!
I have a problem. I'm analyzing glycerin and carbohydrates in creams by HPLC using RI and anionic column (Hamilton RCX-10).
Chromatograms of Standards are Ok. If I inject several times the same sample of cream, peaks of carbohydrates mantain the same area but not the peak of glycerin. In chromatogram of later injection of water appears glycerin peak (ghost peak?).
I use speed cartridge octadecyl C18/18% and 0.2um filters.
What's wrong?
Thank you.
I have a problem. I'm analyzing glycerin and carbohydrates in creams by HPLC using RI and anionic column (Hamilton RCX-10).
Chromatograms of Standards are Ok. If I inject several times the same sample of cream, peaks of carbohydrates mantain the same area but not the peak of glycerin. In chromatogram of later injection of water appears glycerin peak (ghost peak?).
I use speed cartridge octadecyl C18/18% and 0.2um filters.
What's wrong?
Thank you.
