Definition of impurity

[dRima]

Hello!

I am beginner, only start to understand all processes and spectrometry subtleties. Therefore in something I can be mistaken!

Before me there is now a problem definition of impurity in pesticidal preparations. It can be solvents, dyes etc. Research is spent on TOF-MS detector (Q-star Elite), ESI. A mobile phase 0,1%HCOOH/CH3CN=50/50
Flow = 0,2 ml/min
Tell me please, in what way I can define or at least see 1-Butanol? I try to carry out the analysis with a column and without. Do not see I peak m/z=75 (((

Can be it is necessary to use a column with a gradient? What column preferable to such analyses?

Whether there is for someone an experience of such researches? (Research of impurity)
Thank you very much!

[Peter Apps]

Butanol will be much more easily done by GC than by LC.

Peter

[dRima]

Thank you for your reply!
I understand that GC is more convenient! But the work is only used LC / TOF-MS.

[tom jupille]

The first thing to do would be to set up an infusion experiment where you pump a dilute solution of butanol in your mobile phase directly into the interface. Either monitor at m/z=75 or scan around that value and tune your ESI parameters until you get a signal. That will establish that the MS part of your system is working. Only then should you worry about the LC part (isocratic or gradient).

[dRima]

Ок, Tom Jupille.
Thank you very much!
I will try tomorrow to get a signal by changing the settings ESI. Understand that the first i must just get a signal to the MS, and then had to think about LC parametres.