thc metabolite

[che313]

thc-cooh (carboxylic acid) and similar components like steroids as I know It must be derivatized by bstfa or whatever so it can be detected by gc-ms. but in lc/ms it is not necessary to derivatization.

now, in gc-ms, if i run isothermally at 120C, it wouldn't reach the ms? and then can be ionised and detected?

[tom jupille]

Derivatisation with BSTFA is not done to make organic compounds detectable, it is done to make them volatile and thermally stable enough to get through the GC.