Water in PEG column

[chhubert]

I am wondering whether it's still damaging the bonded PEG capillary column when injecting aqueous sample into it, 'cos as far as I know this kind of column is prone to water and oxygen.

[Consumer Products Guy]

All depends upon "how much" (how much water, how much oxygen, how much heat, how much time at that temperature). We've made 0.5 ul water injections for over a decade on a PEG column (Supelco Nukol) for alcohol assays at 40C with no issues. Prior to a sequence we take up to 150C.

[Ron]

Air is more damaging to a bonded phase wax column that water is. As long as you keep the system airtight you can do a lot of water injections without damaging the column. The column might last longer if you don't do water injections, but the difference wouldn 't be significant. Remember, a column is considered a consumable item, they aren't designed to last forever.