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CAM Column for Glycerol
Posted: Fri Sep 18, 2009 3:47 pm
by TimGG
Does anyone know is it possible to use GC-FID CAM column to detect glycerol??
If it is possible, can you tell me what's the setting on the GC?? I'm using Aglient 7890A, thanks for your help!!
Posted: Fri Sep 18, 2009 6:00 pm
by chromatographer1
My guess would be another phase might work better.
Glycerol is too acidic for the column you are using.
Perhaps, a FFAP column might work better, or an ordinary Wax column.
best wishes,
Rodney George
consultant
Posted: Sat Sep 19, 2009 7:49 am
by TimGG
Thanks Rodney, I know I should pick a Wax column but just hoping it works~~~ but really appreciated
Posted: Sat Sep 19, 2009 3:24 pm
by Consumer Products Guy
The "624" columns (e.g. Rtx-624) detailed in the USP monograph (for impurities in glycerin) give a decent peak shape for underivatized glycerin.
At my work we've assayed for glycerin levels in consumer products and raw materials for decades by GC on non-polar capillaries after trimethylsilyl derivatization.
Posted: Mon Sep 21, 2009 2:47 pm
by AICMM
Sorry, for newbies like me, could someone explain what a CAM column is? Just curious.
Best regards.
Posted: Mon Sep 21, 2009 4:07 pm
by chromatographer1
A CAM column is a carbowax phase column with the surface/phase deactivated to amines. It has a more basic character than a ordinary Carbowax (PEG) phase like the FFAP phase has a more acidic character than the Carbowax phase, although it too is based on Carbowax.
Rodney George
consultant