Sample loop in Waters 717plus autosampler

[chhubert]

Dear all,

I want to change the sample loop of the Waters 717plus autosampler to 100uL in order to minimize the system dead volume. Is there any one who has such practical experience ? For Waters 717plus autosampler, there are only two sizes of sample loop - 200 and 2000uL. Thanks a lot.

Hubert

[danko]

Just remove the existing loop (200 µL ?) and then install the 100 µL loop (the fittings are the same)

Best Regards

[AA]

Simply replacing the loop will not reduce the volume enough to make a significant impact on the chromatography. The option is to install the Low Volume Stainless Steel Tubing Kit [WAT055886] for the 717.

[chhubert]

AA: I have replaced the low volume stainless steel tubing kit already. However, I find that it only reduces the system dead volume between the pump and the autosampler but not the autosampler (or sample loop) to column.
Danko: In the front panel of 717plus, I can only select the sample loop of 200uL or 2000uL. Will there be any problem (instrument-wise) if I change the loop physically to 100uL but keep the sample loop setting on the panel to 200uL?
Thanks a lot.

Hubert

[danko]

No problem at all. Just choose 200 µL. It works perfectly with 100 µL loop.

Best Regards

[chhubert]

Thanks a lot, Danko. I'd try and see how it improves my work.

Hubert