peak area count range

[cygg168]

For waters HPLC UV detector, what peak area count range is considered as normal for an active peak? Is there a general rule for the assay level and impurity level?

Thank you for your help.

[shahis77]

No there is no specific limit for the peak area but there are limits for signal to noise..

[LC_labrat]

cygg168-

Generally most peak areas should be within 2.0%RSD and impurities 5-10%RSD, depending on the quantitation levels.

[small dogtail]

For waters HPLC UV detector, what peak area count range is considered as normal for an active peak? Is there a general rule for the assay level and impurity level?

Thank you for your help.

My personal experience:

Assay: UV absorance <1.5 absorance Unit, typically 0.6-0.8
Area: 200~5000

Impurity level:
peak area <200