Relationship between d0, Rs* and relative peak height

[Rob Burgess]

Hi there I was wondering if any mathematically inclined persons can help me with a problem of visualising this.

I'm tring to construct (for training purposes) a plot in Excel from 1st principles that shows the relationship between d0 (the discrimination factor) Rs* (critical resolution to see a peak emerging as a valley) and the relative peak heights (hrp).

I've seen the following equation equation from Uwe Neue's HPLC Columns book:

d0 = 1 -2exp(-2Rs^2)

and would like to construct my own plots similar to that shown in Fig 16.2 of the the book or Fig 2 of the following paper: M. El Fallah & M. Martin, Discrimination Factor and effective peak capacity, Chromatographia, Vol. 24 (1987)115.

However I don't know where the (hrp) fits into the grand scheme of things and thus how to contruct the corrct plots. Can anyone help, it would be much appreciated .... cheers

[Uwe Neue]

I am not sure what you would like. A peak simulator that can create peaks of different Rs values and heights? I can send you a rather user-friendly spreadsheet that can do that. Contact me either at Uwe_Neue@Waters.com or at Uwe.Neue@Prodigy.net.

Uwe

[grzesiek]

althought we would like to help it's difficult to understand what you want, please bear in mind that not everybody has access to the journal and Uwe's book