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Issues with LC-UV spectra Chromeleon

Posted: Tue Apr 14, 2020 9:16 am
by kate_m
hi everybody

is anybody familiar with chromeleon?

i have a sudden issue with my UV data where it shows literally everything so i cant identify any of my peaks - see image 1.

Image

see image 2 for what my uv spectra used to look like for the same peak - it is a control sample from a different sequence.

Image

I am unable to assign peaks using my spectral database either as he doesn't match to the peak he should. I am certain what this peak is and what he should match with because it is a spiked sample.

The spectra sampling points are blanked out if i right click > properties - see image 3.

Image

Can anybody help?

kate

Re: Issues with LC-UV spectra Chromeleon

Posted: Thu Apr 23, 2020 5:08 pm
by pwm
Looks like the peak you want is at 211nm from the good spectrum? That appears to be missing from the bad one. Do you have any 2D chromatogram comparisons?