Issues with LC-UV spectra Chromeleon

[kate_m]

hi everybody

is anybody familiar with chromeleon?

i have a sudden issue with my UV data where it shows literally everything so i cant identify any of my peaks - see image 1.



see image 2 for what my uv spectra used to look like for the same peak - it is a control sample from a different sequence.



I am unable to assign peaks using my spectral database either as he doesn't match to the peak he should. I am certain what this peak is and what he should match with because it is a spiked sample.

The spectra sampling points are blanked out if i right click > properties - see image 3.



Can anybody help?

kate

[pwm]

Looks like the peak you want is at 211nm from the good spectrum? That appears to be missing from the bad one. Do you have any 2D chromatogram comparisons?