HPLC Silica column problems

[SIELC_Tech]

Here are links for your type of compounds (adenine, adenosine, cysteine). Retention time is well controlled by amount and nature of buffer and amount of acetonitrile.

http://www.sielc.com/compound_030.html#
http://www.sielc.com/compound_025.html
http://www.sielc.com/application_013.html

I have couple files for serine and homoserine I posted it on Chromforum while ago and you can do a search.

Contact us if you need more information.

Regards,

Vlad

[Uwe Neue]

If you do not have phosphoric acid, use another strong acid that does not eat your HPLC instrument (do not use HCl, nitric is OK, but phosphoric is best).

Let us observe afterwards what is happening with the retention in your standard mobile phase. The addition of acetic acid is not a good idea, since you need to detect at 210. Your detector would go blind for the blah compound. If it is reasonably stable we stick with what we have got. If not, I'll scratch my head again.

[rekuci]

Fortunately we have an old crusty bottle of phosphoric acid. I'll keep you posted what happens.

[Kostas Petritis]

Maybe if it is too old, you shouldn't use it