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fraction collection

http://www.chromforum.org/viewtopic.php?t=24495

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Re: fraction collection

Posted: Tue Apr 08, 2014 8:34 pm
by Hollow
I'm a fan of theoretical thinking myself, but in this case, why not just collect timed fractions from let's say 64.5 - 69 min (about 2 ml) in reasonable small windows, reanalyze them and see where the substance is collected? Afterwards programm the collector accordingly.

Re: fraction collection

Posted: Thu Apr 10, 2014 12:05 pm
by LCbob
Hi
Most fraction collectors have a 2 or 3 way solenoid valve to pass the eluent from waste to collector. Just check when the system is in collect mode there is no eluent going to waste.
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